Mastery course · Advanced Mathematics and Statistics for Perfumers

Discrimination Tests and Thurstonian d′

Derive the binomial test behind a triangle test, convert a proportion correct into d′, and see why d′ compares methods where proportion correct cannot.

In short

A triangle test's result is a count of correct answers, and its significance is an exact binomial calculation against a guessing rate of one third. But the proportion correct depends on the method, so it cannot compare a triangle test with a paired test. The Thurstonian model converts it into d′, the distance between two products' sensation distributions, which belongs to the products rather than the method — and which is what a sample-size calculation should be built on.

What you will learn

  • Compute the exact binomial p-value for a triangle test and find the critical number correct
  • Calculate the proportion of discriminators and explain its guessing model
  • Explain the Thurstonian model and convert a proportion correct into d′ for the triangle and 2-AFC methods
  • Choose a method and sample size for a stated effect, using d′

Before this lesson: Sample Size and Sensory Statistics, Controls, Randomization and Blinding, Comparative Evaluation: Choosing the Right Test

Before you start

You need sample size and power, blinding and randomization and comparative evaluation.

The binomial test

Under the null hypothesis of no perceptible difference, each of n assessors picks the odd sample with probability p0 = 1/3. The probability of at least k correct is

P(X ≥ k) = Σi=kn C(n, i) p0i (1 − p0)n−i

For n = 36: P(X ≥ 17) ≈ 0.058 and P(X ≥ 18) ≈ 0.028, so 18 correct is the smallest significant count at α = 0.05, matching ISO 4120's table. A result of 20 correct gives P ≈ 0.005.

The guessing model and its weakness

The proportion of discriminators assumes each assessor either truly perceives the difference (and is always right) or guesses:

pd = (pc − p0) / (1 − p0)

With 20/36 correct, pc = 0.556 and pd = 0.333: "a third of the population discriminates". This is intuitive and widely reported, but its picture of perception — everyone either perceives perfectly or not at all — is not how sensation works, and pd for the same products changes with the method.

The Thurstonian model

Each presentation of a product gives a sensation drawn from a normal distribution; two products differ by d′ standard deviations. A method is a decision rule applied to these noisy sensations, and its proportion correct is a known function of d′. For the 2-AFC (which is stronger?) it is simply

pc = Φ(d′ / √2)

where Φ is the standard normal distribution function. For the triangle test the function is an integral with no closed form, usually read from tables; integrated numerically, d′ = 1 gives pc ≈ 0.42 and d′ = 2 gives ≈ 0.61.

One pair of fragrances, two methods (computed from the models)
QuantityTriangle2-AFC
Observed pc0.556—
Implied d′≈ 1.75same products, so ≈ 1.75
Predicted pc0.556≈ 0.89

The triangle test is a hard cognitive task — finding the odd one of three — so even a large d′ yields a modest proportion correct. A directional 2-AFC, where the attribute is named, uses the same sensory difference far more efficiently. That is the main practical consequence: for a known attribute (is the reformulation less animalic?), a directional test needs far fewer assessors for the same d′.

Planning with d′

State the smallest difference that matters as a d′ — say, a reformulation must not reach d′ = 1 — then choose the method and calculate the sample size that would detect that d′ with your chosen power. Ennis's 1993 paper gives such tables; later work notes that its normal approximation overstated power slightly, so check with exact binomial calculations.

Where it stops

  • Equal-variance normal sensations are an assumption. Fragrance evaluation adds adaptation and carryover, which the basic model ignores.
  • Failing to reject is not proving similarity. A non-significant triangle test with twelve people does not show the products are the same; a similarity test is designed differently, around a maximum tolerable d′.
  • Assessors are not interchangeable. Trained evaluators and consumers have different d′ for the same products.

Open questions

How large a d′ do consumers notice in use, as opposed to in a lab test? Linking laboratory d′ to in-use detection for fragrance is still a research question, and it decides how strict a reformulation's target should be.

Practical exercise: Plan and analyze a reformulation test with d′

About 180 minutes · You will have: a test plan stating the method, d′ of concern and sample size, the analysis of a run test with binomial p-value, proportion of discriminators and d′, and a decision statement

  1. Choose a reformulation question: an original and a cost-reduced version of one accord.
  2. Write the plan before testing: method (triangle or directional), the d′ you care about, α, desired power and the sample size you derive from published tables or your own binomial calculation.
  3. Run the test blinded and randomized, with as many people as you can honestly recruit.
  4. Report: number correct, exact binomial p-value, pd, and d′ (from tables or numerical integration), with a sentence on what the result does and does not establish.

You have done this well if the plan was written before the test, your p-value is the exact binomial one, your d′ is computed for the method you actually used, and the decision statement says whether the test could have detected the difference you care about.

Common mistakes

  • Comparing pc across methods. Compare d′.
  • Calling a non-significant result "no difference". Check power first.
  • Testing too few people and concluding anything strong. Small panels can only detect large d′.

Key terms

Thurstonian model
A model of discrimination in which each sample produces a sensation that varies randomly from moment to moment. Difference is measured as the distance between the two distributions in standard-deviation units, d′.
d′ (d-prime)
The standardized distance between the sensation distributions of two products. It describes the products' difference independently of the test method used to measure it.
Proportion of discriminators
pd = (pc − p0)/(1 − p0), where pc is the proportion correct and p0 the guessing probability. It assumes each assessor either discriminates or guesses, which the Thurstonian model does not.
2-AFC test
Two-alternative forced choice: two samples, and the assessor names the one that is stronger in a specified attribute. Guessing gives 1/2.

Knowledge check

1. In a triangle test with 36 assessors, how many correct answers are needed for significance at α = 0.05 (one-sided)?
Show the answer

18. P(X ≥ 18 | n = 36, p = 1/3) ≈ 0.028 while P(X ≥ 17) ≈ 0.058, so 18 is the critical number; ISO 4120's table agrees.

2. 20 of 36 assessors are correct in a triangle test. What is the proportion of discriminators?
Show the answer

0.33. pd = (20/36 − 1/3)/(2/3) = (0.556 − 0.333)/0.667 = 0.333.

3. The same pair of fragrances gives pc = 0.56 in a triangle test. Roughly what d′ is that, and what would a 2-AFC test give?
Show the answer

d′ ≈ 1.75; a 2-AFC would give pc ≈ 0.89. The triangle is a hard task: a large d′ produces a modest pc. The same d′ in the easier, directional 2-AFC gives a much higher proportion correct.

4. Why is d′ a better basis than pd for comparing results across methods?
Show the answer

It is a property of the products, while pd depends on the method's guessing rate and task. The same products yield different pc and pd under different methods; d′ is the method-independent quantity.

Written and reviewed by the RUŌOD Lab team. This is general education, not legal, regulatory, medical or safety advice. Example dilutions and exercises are for learning, are not validated products, and have not been safety-assessed. Completing a lesson confers no qualification. How we check these lessons.